Fifteen pre-weighed stainless-steel coupons were soiled with synthetic blood using a swab and allowed to air dry for 24 hours before recording dirty weights. Five cleaners were prepared using the specified dilutions and temperature: Super Nova.1 NpHD (0.8ml/1L at 160F), Biogone (33% dilution at room temperature 68 F), Pentazyme (0.5 oz/gal at 140 F), and PentaPrep Spray (RTU at 140 F). Three coupons per cleaner were immersed in a beaker for two minutes except for Biogone, which had a three-minute contact time. Coupons were rinsed in a DI water bath and dried for 24 hours before recording clean weights.