Bigone concentration: 0.25oz/gal was tested at three temperatures: 25C, 50C, and 60C. Three stainless steel coupons were chosen for each temperature tested for a total of nine coupons. Each coupon was weighed and had their initial weights recorded. The coupons were then soiled with the provided synthetic blood by using a swab to wipe the blood on the lower third of each coupon (where the coupon would come in contact with the cleaning agent during immersion). The coupons were left to air dry for 24 hours before having their dirty weights recorded. The coupons were then subjected to 2 minutes of immersion in the Biogone 0.25oz/gal at their respective temperatures with a stir bar set to 200 rpm. After cleaning, each coupon was rinsed in a DI water bath for 1 minute with a stir bar set to 200 rpm. The coupons were left to air dry overnight before having their clean weights recorded.