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Trial #1

Trial #1

The objective of this study was to determine the effectiveness of commercially available steam devices for disinfection

2014

21

2

0

0

Coupon

03/06/2014

1.00

Steam

Alicia Melvin

Ceramics
Plastic
Stainless Steel

There are no related objects.

None

Air dry

Visual

All tests performed in triplicate with pos and neg controls. 
All tests were performed with a 30 second contact time.
All tests were performed with S. aureus and E. coli.
Tests were performed on aluminum and stainless steel.
Towel variations included: Microfiber washed with no fabric softener
TNTC= Too Numerous to Count (>300 Colonies)

Lab Methods
Day 1
• Subculture Bacteria and Prepare Supplies.
o Make Tryptic Soy Agar Plates
o Autoclave Supplies
o Gather Equipment

Day 2
• Conduct Run
o Set up Biological Safety Cabinet
o Spot Coupon Surface with Inoculation, plate Standardized Inoculum.
o Prepare Controls
o Test Cleaning
o Wrist Action Shaker
o Dilutions
o Plate

Day 3
• Count Plates
o Determine Effectiveness

Organism Coupon Test Dilution Result Duplicate result
S. aureus Aluminum Negative 1:1 2 0
S. aureus Aluminum Positive 1:1 TNTC TNTC
S. aureus Aluminum Positive 1:10 TNTC TNTC
S. aureus Aluminum Positive 1:100 50 63
S. aureus Aluminum Positive 1:1000 2 2
S. aureus Aluminum Standard In. 10-9 TNTC TNTC
S. aureus Aluminum Standard In. 10-10 TNTC TNTC
S. aureus Aluminum Standard In. 10-11 188 180
S. aureus Aluminum Standard In. 10-12 108 95
S. aureus Aluminum 1 1:1 0 0
S. aureus Aluminum 1 1:10 1 0
S. aureus Aluminum 1 1:100 1 0
S. aureus Aluminum 1 1:1000 1 0
S. aureus Aluminum 2 1:1 0 0
S. aureus Aluminum 2 1:10 1 0
S. aureus Aluminum 2 1:100 1 0
S. aureus Aluminum 2 1:1000 3 0
S. aureus Aluminum 3 1:1 1 0
S. aureus Aluminum 3 1:10 2 2
S. aureus Aluminum 3 1:100 0 0
S. aureus Aluminum 3 1:1000 1 0

.

Organism Coupon Test Dilution Result Duplicate result
S. aureus Aluminum Negative 1:1 2 0
S. aureus Aluminum Positive 1:1 TNTC TNTC
S. aureus Aluminum Positive 1:10 TNTC TNTC
S. aureus Aluminum Positive 1:100 50 63
S. aureus Aluminum Positive 1:1000 2 2
S. aureus Aluminum Standard In. 10-9 TNTC TNTC
S. aureus Aluminum Standard In. 10-10 TNTC TNTC
S. aureus Aluminum Standard In. 10-11 188 180
S. aureus Aluminum Standard In. 10-12 108 95
S. aureus Aluminum 1 1:1 0 0
S. aureus Aluminum 1 1:10 1 0
S. aureus Aluminum 1 1:100 1 0
S. aureus Aluminum 1 1:1000 1 0
S. aureus Aluminum 2 1:1 0 0
S. aureus Aluminum 2 1:10 1 0
S. aureus Aluminum 2 1:100 1 0
S. aureus Aluminum 2 1:1000 3 0
S. aureus Aluminum 3 1:1 1 0
S. aureus Aluminum 3 1:10 2 2
S. aureus Aluminum 3 1:100 0 0
S. aureus Aluminum 3 1:1000 1 0

.

Organism Coupon Test Dilution Result Duplicate result
S. aureus Aluminum Negative 1:1 0 0
S. aureus Aluminum Positive 1:1 TNTC TNTC
S. aureus Aluminum Positive 1:10 TNTC TNTC
S. aureus Aluminum Positive 1:100 TNTC TNTC
S. aureus Aluminum Positive 1:1000 TNTC TNTC
S. aureus Aluminum Standard In. 10-9 TNTC TNTC
S. aureus Aluminum Standard In. 10-10 TNTC TNTC
S. aureus Aluminum Standard In. 10-11 TNTC TNTC
S. aureus Aluminum Standard In. 10-12 TNTC TNTC
S. aureus Aluminum 1 1:1 0 0
S. aureus Aluminum 1 1:10 0 0
S. aureus Aluminum 1 1:100 0 0
S. aureus Aluminum 1 1:1000 0 0
S. aureus Aluminum 2 1:1 0 0
S. aureus Aluminum 2 1:10 1 2
S. aureus Aluminum 2 1:100 0 0
S. aureus Aluminum 2 1:1000 0 0
S. aureus Aluminum 3 1:1 0 0
S. aureus Aluminum 3 1:10 0 4
S. aureus Aluminum 3 1:100 0 0
S. aureus Aluminum 3 1:1000 0 0

.

Organism Coupon Test Dilution Result Duplicate result
S. aureus Stainless Steel Negative 1:1 0 0
S. aureus Stainless Steel Positive 1:1 TNTC TNTC
S. aureus Stainless Steel Positive 1:10 TNTC TNTC
S. aureus Stainless Steel Positive 1:100 36 33
S. aureus Stainless Steel Positive 1:1000 5 3
S. aureus Stainless Steel Standard In. 10-9 TNTC TNTC
S. aureus Stainless Steel Standard In. 10-10 TNTC TNTC
S. aureus Stainless Steel Standard In. 10-11 TNTC TNTC
S. aureus Stainless Steel Standard In. 10-12 TNTC TNTC
S. aureus Stainless Steel 1 1:1 0 0
S. aureus Stainless Steel 1 1:10 0 0
S. aureus Stainless Steel 1 1:100 0 0
S. aureus Stainless Steel 1 1:1000 0 0
S. aureus Stainless Steel 2 1:1 TNTC TNTC
S. aureus Stainless Steel 2 1:10 TNTC TNTC
S. aureus Stainless Steel 2 1:100 238 202
S. aureus Stainless Steel 2 1:1000 19 31
S. aureus Stainless Steel 3 1:1 TNTC TNTC
S. aureus Stainless Steel 3 1:10 TNTC TNTC
S. aureus Stainless Steel 3 1:100 83 78
S. aureus Stainless Steel 3 1:1000 13 15

.

Organism Coupon Test Dilution Result Duplicate result
E. coli Stainless Steel Negative 1:1 0 0
E. coli Stainless Steel Positive 1:1 TNTC TNTC
E. coli Stainless Steel Positive 1:10 TNTC TNTC
E. coli Stainless Steel Positive 1:100 172 178
E. coli Stainless Steel Positive 1:1000 14 28
E. coli Stainless Steel Standard In. 10-8 TNTC TNTC
E. coli Stainless Steel Standard In. 10-9 TNTC TNTC
E. coli Stainless Steel Standard In. 10-10    
E. coli Stainless Steel Standard In. 10-11 140 163
E. coli Stainless Steel 1 1:1 0 0
E. coli Stainless Steel 1 1:10 0 0
E. coli Stainless Steel 1 1:100 0 0
E. coli Stainless Steel 1 1:1000 0 0
E. coli Stainless Steel 2 1:1 TNTC TNTC
E. coli Stainless Steel 2 1:10 TNTC TNTC
E. coli Stainless Steel 2 1:100 TNTC TNTC
E. coli Stainless Steel 2 1:1000 55 61
E. coli Stainless Steel 3 1:1 TNTC TNTC
E. coli Stainless Steel 3 1:10 TNTC TNTC
E. coli Stainless Steel 3 1:100 TNTC TNTC
E. coli Stainless Steel 3 1:1000 152 Plate Dropped

• Shark
o Versatile
o Tested on Hardwood, Tile, Granite, Baseboards, and Walls
o Temperature Control
o Easy Lift Out Hand Unit
o Water Reservoir Easy to Fill, Lasts
o Awkward Plug on Side of Unit

• Orek
o Floor Unit Only
o Feels “Cheap/Flimsy” to Use

No relation

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Document Evaluation #0 Evaluation 3
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