Project #2

Project #2

Characterization of microbial activity by measuring protein reduction over time by Bradford assay using Pollet's Bacillus subtilis strain and BSA fraction V as the protein.

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09/01/2021

There are no related objects.

Solution

Bacterial Strain

Bacillus subtilis strain; BSA fraction V

Small

Less than 1 lb

Rough

Hard

Simple

No

Bovine Serum Albumin Fraction V (BSA Fraction V)

Yes

Immersion/Soak

Pollet

Time (min)

Tap

None

Purpose of test was to see if their bacterial strain ate the protein food source overtime

BSA is a protein, mimicking dirty conditions. In different standards by Pollet, a Bovine Albumin or a mix BSA + sheep erythrocytes as the interfering substance to reproduce dirty conditions. Pollet requested to use BSA as the protein source in Bradford Assay.

None

None

Used Immediately

room temperature (22.5°C +/- 2.5°C) with shaking the tube at 150 rpm

3 weeks

Time-tested Colorimetric Assay (e.g. Bradford Protein Assay)

Modification of Bradford Protein Assay

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram
quantities of protein utilizing the principle of protein-dye binding. Analytical
Biochemistry, 72(1), 248-254.

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No

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Yes

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