Browse Client Types

Browse past lab clients by general industry sectors

Trial Number 1

Client Number 380

Project Number 1

Trial Purpose:

The objective of this study was to determine the effectiveness of commercially available steam devices for disinfection

Date Run:

03/07/2014

Experiment Procedure:

All tests performed in triplicate with pos and neg controls. 
All tests were performed with a 30 second contact time.
All tests were performed with S. aureus and E. coli.
Tests were performed on aluminum and stainless steel.
Towel variations included: Microfiber washed with no fabric softener
TNTC= Too Numerous to Count (>300 Colonies)

Lab Methods
Day 1
• Subculture Bacteria and Prepare Supplies.
o Make Tryptic Soy Agar Plates
o Autoclave Supplies
o Gather Equipment

Day 2
• Conduct Run
o Set up Biological Safety Cabinet
o Spot Coupon Surface with Inoculation, plate Standardized Inoculum.
o Prepare Controls
o Test Cleaning
o Wrist Action Shaker
o Dilutions
o Plate

Day 3
• Count Plates
o Determine Effectiveness

Trial Results:

Organism Coupon Test Dilution Result Duplicate result
S. aureus Aluminum Negative 1:1 2 0
S. aureus Aluminum Positive 1:1 TNTC TNTC
S. aureus Aluminum Positive 1:10 TNTC TNTC
S. aureus Aluminum Positive 1:100 50 63
S. aureus Aluminum Positive 1:1000 2 2
S. aureus Aluminum Standard In. 10-9 TNTC TNTC
S. aureus Aluminum Standard In. 10-10 TNTC TNTC
S. aureus Aluminum Standard In. 10-11 188 180
S. aureus Aluminum Standard In. 10-12 108 95
S. aureus Aluminum 1 1:1 0 0
S. aureus Aluminum 1 1:10 1 0
S. aureus Aluminum 1 1:100 1 0
S. aureus Aluminum 1 1:1000 1 0
S. aureus Aluminum 2 1:1 0 0
S. aureus Aluminum 2 1:10 1 0
S. aureus Aluminum 2 1:100 1 0
S. aureus Aluminum 2 1:1000 3 0
S. aureus Aluminum 3 1:1 1 0
S. aureus Aluminum 3 1:10 2 2
S. aureus Aluminum 3 1:100 0 0
S. aureus Aluminum 3 1:1000 1 0

.

Organism Coupon Test Dilution Result Duplicate result
S. aureus Aluminum Negative 1:1 2 0
S. aureus Aluminum Positive 1:1 TNTC TNTC
S. aureus Aluminum Positive 1:10 TNTC TNTC
S. aureus Aluminum Positive 1:100 50 63
S. aureus Aluminum Positive 1:1000 2 2
S. aureus Aluminum Standard In. 10-9 TNTC TNTC
S. aureus Aluminum Standard In. 10-10 TNTC TNTC
S. aureus Aluminum Standard In. 10-11 188 180
S. aureus Aluminum Standard In. 10-12 108 95
S. aureus Aluminum 1 1:1 0 0
S. aureus Aluminum 1 1:10 1 0
S. aureus Aluminum 1 1:100 1 0
S. aureus Aluminum 1 1:1000 1 0
S. aureus Aluminum 2 1:1 0 0
S. aureus Aluminum 2 1:10 1 0
S. aureus Aluminum 2 1:100 1 0
S. aureus Aluminum 2 1:1000 3 0
S. aureus Aluminum 3 1:1 1 0
S. aureus Aluminum 3 1:10 2 2
S. aureus Aluminum 3 1:100 0 0
S. aureus Aluminum 3 1:1000 1 0

.

Organism Coupon Test Dilution Result Duplicate result
S. aureus Aluminum Negative 1:1 0 0
S. aureus Aluminum Positive 1:1 TNTC TNTC
S. aureus Aluminum Positive 1:10 TNTC TNTC
S. aureus Aluminum Positive 1:100 TNTC TNTC
S. aureus Aluminum Positive 1:1000 TNTC TNTC
S. aureus Aluminum Standard In. 10-9 TNTC TNTC
S. aureus Aluminum Standard In. 10-10 TNTC TNTC
S. aureus Aluminum Standard In. 10-11 TNTC TNTC
S. aureus Aluminum Standard In. 10-12 TNTC TNTC
S. aureus Aluminum 1 1:1 0 0
S. aureus Aluminum 1 1:10 0 0
S. aureus Aluminum 1 1:100 0 0
S. aureus Aluminum 1 1:1000 0 0
S. aureus Aluminum 2 1:1 0 0
S. aureus Aluminum 2 1:10 1 2
S. aureus Aluminum 2 1:100 0 0
S. aureus Aluminum 2 1:1000 0 0
S. aureus Aluminum 3 1:1 0 0
S. aureus Aluminum 3 1:10 0 4
S. aureus Aluminum 3 1:100 0 0
S. aureus Aluminum 3 1:1000 0 0

.

Organism Coupon Test Dilution Result Duplicate result
S. aureus Stainless Steel Negative 1:1 0 0
S. aureus Stainless Steel Positive 1:1 TNTC TNTC
S. aureus Stainless Steel Positive 1:10 TNTC TNTC
S. aureus Stainless Steel Positive 1:100 36 33
S. aureus Stainless Steel Positive 1:1000 5 3
S. aureus Stainless Steel Standard In. 10-9 TNTC TNTC
S. aureus Stainless Steel Standard In. 10-10 TNTC TNTC
S. aureus Stainless Steel Standard In. 10-11 TNTC TNTC
S. aureus Stainless Steel Standard In. 10-12 TNTC TNTC
S. aureus Stainless Steel 1 1:1 0 0
S. aureus Stainless Steel 1 1:10 0 0
S. aureus Stainless Steel 1 1:100 0 0
S. aureus Stainless Steel 1 1:1000 0 0
S. aureus Stainless Steel 2 1:1 TNTC TNTC
S. aureus Stainless Steel 2 1:10 TNTC TNTC
S. aureus Stainless Steel 2 1:100 238 202
S. aureus Stainless Steel 2 1:1000 19 31
S. aureus Stainless Steel 3 1:1 TNTC TNTC
S. aureus Stainless Steel 3 1:10 TNTC TNTC
S. aureus Stainless Steel 3 1:100 83 78
S. aureus Stainless Steel 3 1:1000 13 15

.

Organism Coupon Test Dilution Result Duplicate result
E. coli Stainless Steel Negative 1:1 0 0
E. coli Stainless Steel Positive 1:1 TNTC TNTC
E. coli Stainless Steel Positive 1:10 TNTC TNTC
E. coli Stainless Steel Positive 1:100 172 178
E. coli Stainless Steel Positive 1:1000 14 28
E. coli Stainless Steel Standard In. 10-8 TNTC TNTC
E. coli Stainless Steel Standard In. 10-9 TNTC TNTC
E. coli Stainless Steel Standard In. 10-10    
E. coli Stainless Steel Standard In. 10-11 140 163
E. coli Stainless Steel 1 1:1 0 0
E. coli Stainless Steel 1 1:10 0 0
E. coli Stainless Steel 1 1:100 0 0
E. coli Stainless Steel 1 1:1000 0 0
E. coli Stainless Steel 2 1:1 TNTC TNTC
E. coli Stainless Steel 2 1:10 TNTC TNTC
E. coli Stainless Steel 2 1:100 TNTC TNTC
E. coli Stainless Steel 2 1:1000 55 61
E. coli Stainless Steel 3 1:1 TNTC TNTC
E. coli Stainless Steel 3 1:10 TNTC TNTC
E. coli Stainless Steel 3 1:100 TNTC TNTC
E. coli Stainless Steel 3 1:1000 152 Plate Dropped

• Shark
o Versatile
o Tested on Hardwood, Tile, Granite, Baseboards, and Walls
o Temperature Control
o Easy Lift Out Hand Unit
o Water Reservoir Easy to Fill, Lasts
o Awkward Plug on Side of Unit

• Orek
o Floor Unit Only
o Feels “Cheap/Flimsy” to Use

Success Rating:

Results successful using TACT (time, agitation, concentration, and temperature, as well as rinsing and drying) and/or other cleaning chemistries examined.

Save Report as a PDF