To evaluate the efficacy of 2-ply toilet paper degradation immersed in a product solution over the span of 10 days.
Date Run:
02/21/2023
Experiment Procedure:
Initial ratings and photos of newly unwrapped 2-ply square toilet paper coupons. The toilet paper coupons were immersed in a solution, either a product/autoclaved minimal media or a control of Deionized (DI) water/ autoclaved minimal media mixture, and incubated at 30°C for 240 hours (10 days). Three evaluators rated the visual degradation at 0hr, 24 hr, 48hr, 144hr, 192hr, and 240hr after shaking the flasks manually for five seconds at each time point using the following rating key:
Rating
Description
1
Completely degraded
2
Mostly degraded
3
Partially degraded
4
Slight degradation
5
No degradation
Protocol for 1L of Minimal Media:
- 7.50 g Bacto peptone media
- 3.27g Bushnell Hass Broth - 1 Liter DI Water
Trial Results:
Over the span of 240 hours (10 days), the Instant Power RV & Marine Holding Tank Treatment increased in turbidity and the degraded toilet paper became black. Similar in turbidity and toilet paper color, the Instant Power Septic Shock and Roebic K-37 Septic Treatment were both visibly degraded by 240 hours. The Roebic RV & Marine Holding Tank Treatment degraded only a little more than the control and was not as cloudy as the other products or control after 192 hours.
Instant Power RV & Marine Holding Tank Treatment
Hour
Evaluation Ratings
Average Rating
0
5
5
5
5
24
5
4.5
4.5
4.7
48
3
3
3
3
144
2.5
2.5
2.5
2.5
192
2
2.5
2.5
2.3
240
1.5
2
2
1.8
Roebic RV & Marine Holding Tank Treatment
Hour
Evaluation Ratings
Average Rating
0
5
5
5
5
24
4
4
4
4
48
4
4
4
4
144
3.5
3.5
3.5
3.5
192
3.5
3.5
3.5
3.5
240
3.5
3.5
3.5
3.5
Instant Power Septic Shock
Hour
Evaluation Ratings
Average Rating
0
5
5
5
5
24
4
4
4
4
48
2
2
2
2
144
1.5
1.5
2
1.7
192
1
1.5
1.5
1.3
240
1
1
1.5
1.2
Roebic K-37 Septic Treatment
Hour
Evaluation Ratings
Average Rating
0
4.5
5
5
4.8
24
3.5
4
4
3.8
48
2.5
2.5
2.5
2.5
144
2.5
2.5
2.5
2.5
192
2
2
2
2
240
1.5
1.5
1.5
1.5
Control (DI Water/Minimal Media)
Hour
Evaluation Ratings
Average Rating
0
5
5
5
5
24
5
5
5
5
48
4.5
4.5
4.5
4.5
144
4.5
4.5
4.5
4.5
192
4.5
4.5
4.5
4.5
240
4.5
4.5
4.5
4.5
Overall, the product with the best 2-ply toilet paper degradation after 240 hours was the Instant Power Septic Shock (1.2), the Roebic K-37 Septic Treatment came in second (1.5), and the Instant Power RV & Marine Holding Tank Treatment (1.8) in third. The least effective product at degrading the 2-ply toilet paper was the Roebic RV & marine Holding Tank Treatment (3.5).
Success Rating:
Results successful using TACT (time, agitation, concentration, and temperature, as well as rinsing and drying) and/or other cleaning chemistries examined.
Conclusion:
Both Instant Power products were more effective than the Roebic comparative products at degrading 2-ply toilet paper over 240 hours.
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